Combinative impact of effectors on production of celluolytic enzyme from Brevibacillus parabrevis ( MTCC 2208 )

A quadratic central composite design (CCD) in response surface methodology was applied to explicate the combinational concentration of effectors that influence fermentative cellulase production by Brevibacillus parabrevis. It was examine that 10% inoculums ((7X10/ml)) concentration and 37C temperature was optimum for cellulase production after 24 h of fermentation. Concentration of effectors (g/100 ml), sodium alginate, 0.3; gum arabica, 0.476; milk whey, 4.75 and Tween 80, 0.85 and pH, 6.5 was optimized, which enhanced enzyme production (126 IU/L) by 2.26 fold on the contrary to the control (48 IU/L). Subsequently supplementation with different carbon source and metal ions also exhibited enhancement in enzyme synthesis.